Mouse Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

Mfr.No.

CSB-EL018969MO

Sensitivity

1.2 ng/mL

Detection Range

4.7 ng/mL-300 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

prostaglandin D2 synthase 21kDa (brain), RP11-229P13.6, L-PGDS, LPGDS, PDS, PGD2, PGDS, PGDS2, PGD2 synthase, beta-trace protein, glutathione-independent PGD synthase, lipocalin-type prostaglandin D synthase, prostaglandin D synthase, prostagla

Recovery

The recovery of mouse IHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	107	103-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	101
	Range %	98-105
1:4	Average %	87
	Range %	84-91
1:8	Average %	100
	Range %	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.785	1.831	1.808	1.687
100	1.181	1.201	1.191	1.070
50	0.737	0.800	0.769	0.648
25	0.455	0.435	0.445	0.324
12.5	0.301	0.312	0.307	0.186
6.25	0.208	0.204	0.206	0.085
3.12	0.167	0.161	0.164	0.043
0	0.122	0.120	0.121	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O09114

Applications	ELISA
Research Area	PTGDS

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506508	CSB-EL018969MO	96T	$695	Add to Cart
BHE10506508	CSB-EL018969MO	5x96T	$3336	Add to Cart
BHE10506508	CSB-EL018969MO	10x96T	$6602.50	Add to Cart

Mouse Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

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