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Home / ELISA Kit

Mouse Prolyl Endopeptidase - PREP ELISA Kit

SKU : BHE12115497

Cleaves Peptide bonds on the C-terminal side of prolyl residues within Peptides that are up to approximately 30 amino acids long.

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Bioassay Technology Laboratory

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Mfr.No.

E2664Mo

Sensitivity

0.016ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PE;Post-proline cleaving enzyme;PREP;Prolyl endopeptidase

Molecular Weight

80752

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2664Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4	0.16	4.1
Intra-Assay	2	18	7	0.35	5
Intra-Assay	3	18	2.4	0.13	5.6

Background

Cleaves Peptide bonds on the C-terminal side of prolyl residues within Peptides that are up to approximately 30 amino acids long.

Protocol

These standard curves of E2664Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	1.813	1.752	1.693
	1.691
4ng/ml	1.042	1.007	0.947
	0.971
2ng/ml	0.639	0.578	0.519
	0.518
1ng/ml	0.198	0.171	0.112
	0.144
0.5ng/ml	0.057	0.074	0.014
	0.09
0ng/ml	0.058	0.059	0
	0.061

Gene ID	19072
UniProt ID	Q9QUR6

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115497	E2664Mo	48T	$320		Add to Cart
BHE12115497	E2664Mo	96T	$458		Add to Cart

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