Mouse Prolactin ELISA Kit PicoKine®

Mfr.No.

EK0594

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.064	0.135	0.209	0.316	0.538	0.887	1.365	1.938

Description

Mouse Prolactin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Prl in cell culture supeatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Prolactin;PRL;Prl;

Molecular Weight

25496 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L32-C228

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Prolactin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	247	1548	4124	254	1402	4314
Standard deviation	13.83	68.11	206.2	15.74	82.71	228.64
CV (%)	4.4%%	4.4%	5%	6.2%	5.9%	5.3%

Protein Function

Prolactin acts primarily on the mammary gland by promoting lactation.

Background

Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	247	250	249	260	251	5.02	2%
Sample 2	1548	1485	1544	1578	1538	33.69	2.1%
Sample 3	4124	4396	4363	4724	4401	213.61	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	19109
Gene Name	PRL
UniProt ID	P06879
Protein Name	Prolactin

Applications	ELISA
Research Area	Growth Factors/Hormones,Lipid Metabolism,Metabolism,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Tyrosine Kinases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000539	EK0594	96 wells/kit	$499		Add to Cart

Mouse Prolactin ELISA Kit PicoKine®

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