Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine®

Mfr.No.

EK1621

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.041	0.141	0.193	0.341	0.598	0.852	1.531	2.056

Description

Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Pon1 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serum paraoxonase/arylesterase 1; PON 1; 3.1.1.2

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A30-L355

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse PON1

Subcellular Localization

Secreted, extracellular space.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	364	1139	5447	343	1198	5787
Standard deviation	21.47	54.67	403.07	25.38	79.06	468.74
CV (%)	7.4%	4.8%	7.4%	7.4%	6.6%	8.1%

Protein Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

Background

Serum paraoxonase/arylesterase 1 (PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	364	310	330	311	328	21.85	6.6%
Sample 2	1139	1282	1324	1250	1248	68.58	5.4%
Sample 3	5447	5579	4941	5080	5261	260.23	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	18979
Gene Name	Pon1
UniProt ID	P52430
Tissue Specificity	Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Diabetes,Diabetes-associated,Drug Metabolism,Heart Disease,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Neurodegenerative Disease,Neurology Process,Neuroscience,Oxidative Stress,Pathways and Processes,Redox Metabolism,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001291	EK1621	96 wells/kit	$499		Add to Cart

Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine®

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