Mouse Polycomb Complex Protein Bmi-1

Mfr.No.

E2018Mo

Description

This sandwich kit is for the accurate quantitative detection of Mouse Polycomb Complex Protein BMI-1 (also known as BMI-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BMI 1;BMI1;Polycomb complex protein BMI-1;Polycomb group RING finger protein 4

Reactivity

Mouse

Detection Range

0.05-30ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36708

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/ml

Background

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).

Gene ID	12151
UniProt ID	P25916
Antigen	BMI1

Research Area

Cell Biology; Epigenetics and Nuclear Signaling; Stem Cells

Protocol

These standard curves of E2018Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.307	2.183	2.129
	2.058
8ng/ml	1.63	1.586	1.532
	1.541
4ng/ml	1.026	1.007	0.954
	0.989
2ng/ml	0.722	0.724	0.67
	0.725
1ng/ml	0.496	0.446	0.393
	0.397
0ng/ml	0.062	0.054	0
	0.045

Precision

We measured random samples of E2018Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.43	0.33	4.41
Intra-Assay	2	18	3.63	0.21	5.76
Intra-Assay	3	18	1.99	0.11	5.55

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109829	E2018Mo	48T	$320		Add to Cart
BHE12109829	E2018Mo	96T	$458		Add to Cart

Mouse Polycomb Complex Protein Bmi-1 - BMI-1 ELISA Kit

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