Mouse PLUNC(Palate/Lung And Nasal Epithelium Associated Protein) ELISA Kit

Mfr.No.

ELK6635

Sensitivity

6.9 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

BPIFA1; SPURT; NASG; LUNX; SPLUNC1; Secretory Protein In Upper Respiratory Tracts; BPI Fold Containing Family A, Member 1; Nasopharyngeal carcinoma-related

Recovery

Matrices listed below were spiked with certain level of recombinant PLUNC and the recovery rates were calculated by comparing the measured value to the expected amount of PLUNC in samples.

Matrix	Recovery range	Average
serum(n=5)	89-107%	98%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	78-90%	84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PLUNC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	83-92%	92-96%	86-98%	95-101%
EDTA plasma(n=5)	89-98%	81-94%	96-105%	92-103%
Heparin plasma(n=5)	87-98%	92-105%	95-107%	82-96%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.946	1.838
500.00	1.628	1.520
250.00	1.135	1.027
125.00	0.933	0.825
62.50	0.589	0.481
31.25	0.315	0.207
15.63	0.215	0.107
0.00	0.108	0.000

Sample Type

Serum, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PLUNC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PLUNC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PLUNC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PLUNC in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Tumor immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206381	ELK6635	48T	$320		Add to Cart
BHE15206381	ELK6635	96T	$458		Add to Cart

Mouse PLUNC(Palate/Lung And Nasal Epithelium Associated Protein) ELISA Kit

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