Mouse PLGF-2 / PGF ELISA Kit PicoKine®

Mfr.No.

EK0863

Sensitivity

<2 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.034	0.106	0.254	0.383	0.663	1.134	1.880	2.417

Description

Mouse PLGF-2 / PGF ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Pgf in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Placenta growth factor; PlGF; Pgf; Plgf

Immunogen

Expression system for standard: NS0; Immunogen sequence: A24-P158

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse PLGF-2

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	33	176	474	34	182	481
Standard deviation	2.01	12.14	20.38	2.24	13.83	20.68
CV (%)	6.1%	6.9%	4.3%	6.6%	7.6%	4.3%

Protein Function

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth (By similarity). .

Background

Placental growth factor is a protein that in humans is encoded by the PGF gene. Placental growth factor (PGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading mateal arteries. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal skeletal muscle. As a result of alteative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	33	40	36	33	35	2.87	8.2%
Sample 2	176	178	189	176	179	5.4	3%
Sample 3	474	405	411	424	428	27.15	6.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	18654
Gene Name	Pgf
UniProt ID	Q544A5

Applications	ELISA
Research Area	Metabolism,Pathways and Processes,Signal Transduction,Cancer,Metabolism Processes,Cancer Metabolism,Growth Factors/Hormones,Response To Hypoxia

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001300	EK0863	96 wells/kit	$499		Add to Cart

Mouse PLGF-2 / PGF ELISA Kit PicoKine®

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