Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine®

Mfr.No.

EK1829

Sensitivity

<50 pg/ml

Detection Range

93.8 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.066	0.166	0.190	0.301	0.617	1.038	1.642	2.421

Description

Mouse Plexin C1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Plxnc1 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin-C1; Virus-encoded semaphorin protein receptor; CD232; Plxnc1; Vespr

Immunogen

Expression system for standard: NS0; Immunogen sequence: A35-T950

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Plexin C1

Subcellular Localization

Membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	168	824	2614	172	830	2761
Standard deviation	7.2	47.8	162.07	11.35	48.97	209.8
CV (%)	4.3%	5.8%	6.2%	6.6%	5.9%	7.6%

Protein Function

Receptor for SEMA7A, for vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.

Background

A Plexin is a protein which acts as a receptor for Semaphorin family signaling proteins. This gene is mapped to 10; 10 C2. It is classically known for its expression on the surface of axon growth cones and involvement in signal transduction to steer axon growth away from the source of Semaphorin. Plexin also has implications in development of other body systems by activating GTPase enzymes to induce a number of intracellular biochemical changes leading to a variety of downstream effects.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	168	149	160	163	160	6.96	4.3%
Sample 2	824	814	878	902	854	36.67	4.2%
Sample 3	2614	2932	2991	2861	2849	143.54	5.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	54712
Gene Name	Plxnc1
UniProt ID	Q9QZC2
Tissue Specificity	Detected on dendritic cells, skin Langerhans cells and neutrophils.

Applications	ELISA
Research Area	Cancer,Cell Adhesion,Invasion/Microenvironment

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001410	EK1829	96 wells/kit	$750		Add to Cart

Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine®

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