Mouse Plexin A1/PLXNA1 ELISA Kit PicoKine®

Mfr.No.

EK1818

Sensitivity

<0.1 ng/ml

Detection Range

0.312 ng/ml - 20 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	0.3	0.63	1.25	2.5	5	10	20
O.D.	0.158	0.232	0.286	0.326	0.607	0.935	1.409	2.343

Description

Mouse Plexin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Plxna1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin-A1; Plex 1; Plexin-1; Plxna1; Kiaa4053

Immunogen

Expression system for standard: NS0; Immunogen sequence: S28-P1242

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Plexin A1

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	462	2424	8962	500	2463	9301
Standard deviation	21.71	123.62	412.25	27.00	155.17	697.58
CV (%)	4.7%	5.1%	4.6%	5.4%	6.3%	7.5%

Protein Function

Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex compos

Background

Plexin-A1 is a protein that in humans is encoded by the PLXNA1 gene. This gene is mapped to 6; 6 D1. Plexin A1 has been shown to interact with AKT1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	462	417	452	485	454	24.48	5.3%
Sample 2	2424	2827	2492	2766	2627	172.30	6.5%
Sample 3	8962	10130	10185	9716	9748	488.80	5.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	18844
Antigen	A1/PLXNA1
Gene Name	Plxna1
UniProt ID	P70206
Tissue Specificity	Ubiquitous.

Applications	ELISA
Research Area	Axonal Guidance Proteins,Growth and Development,More Gpcr,Neurogenesis,Neurology Process,Neuroscience,Neurotransmission,Receptors / Channels

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001419	EK1818	96 wells/kit	$750		Add to Cart

Mouse Plexin A1/PLXNA1 ELISA Kit PicoKine®

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