Mouse Periostin/OSF2 ELISA Kit PicoKine®

Mfr.No.

EK1187

Sensitivity

<10 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.043	0.115	0.175	0.248	0.446	0.933	1.438	2.042

Description

Mouse Periostin/OSF2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Postn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Periostin;PN;Osteoblast-specific factor 2;OSF-2;Postn;Osf2;

Molecular Weight

93144 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: N24-Q836

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Periostin

Subcellular Localization

Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	109	7119	2510	119	740	2598
Standard deviation	6.97	53.26	110.44	8.33	62.9	119.5
CV (%)	6.4%	6.7%	4.4%	7%	8.5%	4.6%

Protein Function

Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .

Background

Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	109	109	111	107	109	1.41	1.2%
Sample 2	795	840	784	883	825	39.27	4.7%
Sample 3	2510	2434	2494	2308	2436	79.41	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	50706
Gene Name	POSTN
UniProt ID	Q62009
Protein Name	Periostin
Tissue Specificity	Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .
Sequence	Contains 1 EMI domain.

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cardiogenesis,Cardiovascular,Cell Adhesion Molecules,Cell Type Markers,Cytoskeleton/ECM,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Structures,Tags & Cell Markers,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000807	EK1187	96 wells/kit	$499		Add to Cart

Mouse Periostin/OSF2 ELISA Kit PicoKine®

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