Matrices listed below were spiked with certain level of Pde5a and the recovery rates were calculated by comparing the measured value to the expected amount of Pde5a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
97
EDTA plasma(n=5)
88-97
92
heparin plasma(n=5)
90-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pde5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Pde5a ELISA Kit allows for the in vitro quantitative determination of Pde5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Pde5a(cGMP-specific 3 ,5 -cyclic phosphodiesterase) ELISA Kit