Matrices listed below were spiked with certain level of PACAP-38 and the recovery rates were calculated by comparing the measured value to the expected amount of PACAP-38 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
100
EDTA plasma(n=5)
89-103
96
heparin plasma(n=5)
87-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PACAP-38 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PACAP-38 ELISA Kit allows for the in vitro quantitative determination of PACAP-38 concentrations in serum, plasma, tissue homogenates and other biological fluids.