Mouse P16 ELISA Kit

Mfr.No.

E0628Mo

Description

This sandwich kit is for the accurate quantitative detection of Mouse P16 Protein (also known as p16) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Arf;ARF-INK4a;CDK4I;Cyclin-dependent kinase 4 inhibitor A;Cyclin-dependent kinase inhibitor 2A;INK4;Ink4a/Arf;INK4a-ARF;MTS;MTS1;p1;p16;p16(INK4a);p16I;p16-INK4;p16INK4a;p16-INK4a;p19;p19;p19ARF;Pct;Pctr1

Reactivity

Mouse

Detection Range

2-600ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

17941

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.02ng/L

Background

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

Gene ID	12578
UniProt ID	P51480
Antigen	P16ink4a

Research Area

Cell Biology; Epigenetics and Nuclear Signaling; Cancer

Protocol

These standard curves of E0628Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.214	2.274	2.242
	2.334
160ng/L	1.246	1.322	1.29
	1.397
80ng/L	0.863	0.814	0.782
	0.765
40ng/L	0.499	0.503	0.471
	0.506
20ng/L	0.203	0.232	0.2
	0.26
0ng/L	0.024	0.032	0
	0.04

Precision

We measured random samples of E0628Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	120.94	5.97	4.93
Intra-Assay	2	18	216.66	7.71	3.56
Intra-Assay	3	18	60.62	3.76	6.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108506	E0628Mo	48T	$320		Add to Cart
BHE12108506	E0628Mo	96T	$458		Add to Cart

Mouse P16 ELISA Kit

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