Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine®

Mfr.No.

EK2018

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.104	0.147	0.181	0.269	0.424	0.712	1.286	2.230

Description

Mouse Osteoadherin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Omd in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; Osteoadherin; OSAD; Omd

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-I423

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Osteoadherin

Subcellular Localization

Extracellular matrix.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	279	1140	4460	308	1190	4553
Standard deviation	13.39	49.02	182.86	20.33	69.02	346.03
CV (%)	4.8%	4.3%	4.1%	6.6%	5.8%	7.6%

Protein Function

May be implicated in biomineralization processes. Has a function in binding of osteoblasts via the alpha (V)beta3-integrin.

Background

Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 13; 13 A5. Osteoadherin is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	279	280	286	293	284	5.59	1.9%
Sample 2	1140	983	1062	973	1039	67.49	6.4%
Sample 3	4460	4445	3951	4475	4332	220.65	5.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	27047
Gene Name	Omd
UniProt ID	O35103
Protein Name	Osteomodulin
Tissue Specificity	Bone specific.

Applications	ELISA
Research Area	Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Signal Transduction,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001523	EK2018	96 wells/kit	$499		Add to Cart

Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine®

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