Mouse NOPE/IGDCC4 ELISA Kit PicoKine®

Mfr.No.

EK1520

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.031	0.089	0.136	0.269	0.531	1.077	1.476	2.044

Description

Mouse NOPE/IGDCC4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igdcc4 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Immunoglobulin superfamily DCC subclass member 4;Neighbor of punc e11;Protein DDM36;Igdcc4;Ddm36, Kiaa1628, Nope;

Molecular Weight

134765 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-H954

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse NOPE

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	194	1177	4535	182	1179	4547
Standard deviation	13.77	63.55	184.1%3	13.83	84.88	190.97
CV (%)	7.1%	5.4%	4.1%	7.6%	7.2%	4.2%

Background

IGDCC4, also known as NOPE, plays essential roles in biologic functions of the cell surface, including cell adhesion, cell migration, and signal transduction. NOPE is identified as an oncofetal marker of murine and human hepatocellular carcinoma (HCC). Its expression is elevated in mouse and human hepatoma cell lines and in mouse hepatomas, but it is not expressed in normal liver or preneoplastic mouse HCC. Furthermore, NOPE is expressed in both AFP -positive and -negative tumors and is specifically expressed in epithelial tumor cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	194	209	189	183	193	9.62	4.9%
Sample 2	1177	1206	1298	1296	1244	53.74	4.3%
Sample 3	4535	4341	3824	4624	4331	310.08	7.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	56741
Gene Name	Igdcc4
UniProt ID	Q9EQS9
Protein Name	Immunoglobulin superfamily DCC subclass member 4
Tissue Specificity	Expressed in skeletal muscle, heart and brain. Brain expression is hippocampus-specific. .

Applications	ELISA
Research Area	Cell Biology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001105	EK1520	96 wells/kit	$750		Add to Cart

Mouse NOPE/IGDCC4 ELISA Kit PicoKine®

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