Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine®

Mfr.No.

EK1445

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.047	0.118	0.175	0.322	0.640	1.100	1.511	2.055

Description

Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Elane in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neutrophil elastase;3.4.21.37;Elastase-2;Leukocyte elastase;Elane;Ela2;

Molecular Weight

28648 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-R260

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Elane

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	97	301	903	95	322	978
Standard deviation	7.66	17.75	69.53	7.69	19.32	76.28
CV (%)	7.9%	5.3%	7.7%	8.1%	6%	7.8%

Protein Function

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity). .

Background

Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	97	82	82	97	89	7.5	8.4%
Sample 2	301	311	299	294	301	6.17	2%
Sample 3	903	978	956	925	940	28.69	3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	50701
Gene Name	Elane
UniProt ID	Q3UP87
Protein Name	Neutrophil elastase

Applications	ELISA
Research Area	Signal Transduction,Immunology,Microbiology,Cancer,Signaling Pathway,Cytoskeleton/ECM,Calcium Signaling,Extracellular Matrix,Invasion/Microenvironment,ECM Proteins,Innate Immunity,Macrophage/Inflammation,Defensin,Human Protein,Protein,NK Cells,Complement,ECM Enzymes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001057	EK1445	96 wells/kit	$499		Add to Cart

Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine®

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