Mouse Ncan/Neurocan ELISA Kit PicoKine®

Mfr.No.

EK2117

Sensitivity

<30 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.041	0.092	0.164	0.285	0.577	0.964	1.523	2.038

Description

Mouse Ncan/Neurocan ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ncan in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: CHO; Immunogen sequence: D23-D637

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Ncan

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	117	127	1709	118	126	1060
Standard deviation	7.53	9.06	124.59	8.67	3.06	60.1
CV (%)	6.4%	7.1%	7.3%	7.4%	2.4%	5.7%

Background

Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	117	100	106	98	105	8.25	7.9%
Sample 2	127	126	236	140	157	8.82	5.6%
Sample 3	1709	1996	1988	1057	1688	76.11	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	13004
Gene Name	Ncan
UniProt ID	P55066

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001672	EK2117	96 wells/kit	$499		Add to Cart

Mouse Ncan/Neurocan ELISA Kit PicoKine®

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