Mouse Myosin Light Chain Kinase - Smooth Muscle

Mfr.No.

E2281Mo

Alternative Names

Cardiac-MLCK;Cardiac-MyBP-C-associated Ca/CaM kinase;D830007F02Rik;MLCK;MYLK;Myosin light chain kinase 3

Reactivity

Mouse

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

86372

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.41ng/L

Background

Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.

Gene ID	213435
UniProt ID	Q3UIZ8
Antigen	MYLK

Research Area

Others

Protocol

These standard curves of E2281Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.119	2.18	2.122
	2.241
600ng/L	1.174	1.187	1.129
	1.2
300ng/L	0.774	0.774	0.715
	0.774
150ng/L	0.455	0.443	0.384
	0.43
75ng/L	0.214	0.208	0.149
	0.201
0ng/L	0.064	0.059	0
	0.054

Precision

We measured random samples of E2281Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	494.55	24.7	5
Intra-Assay	2	18	678.78	24.11	3.55
Intra-Assay	3	18	208.73	12.51	5.99

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12110092	E2281Mo	48T	$320		Add to Cart
BHE12110092	E2281Mo	96T	$458		Add to Cart

Mouse Myosin Light Chain Kinase - Smooth Muscle - MYLK ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse Myosin Light Chain Kinase - Smooth Muscle - MYLK ELISA Kit

Rate it now..!!