Matrices listed below were spiked with certain level of MPO and the recovery rates were calculated by comparing the measured value to the expected amount of MPO in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
96
EDTA plasma(n=5)
89-105
98
heparin plasma(n=5)
87-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MPO ELISA Kit allows for the in vitro quantitative determination of MPO concentrations in serum, plasma, tissue homogenates and other biological fluids.