JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Mouse Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit

SKU : BHE13705200

Mouse Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UCP1 in samples.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE70038
Reactivity	Mouse
Assay Type	Sandwich
Description	Mouse Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UCP1 in samples.
Detection Method	Colorimetric
Calibration Range	5 pg/mL-80 pg/mL
Limit Of Detection	0.5 pg/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Notes	Mouse Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UCP1 in samples. An antibody specific for UCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Mouse UCP1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of UCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	Mouse Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UCP1 in samples. An antibody specific for UCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Mouse UCP1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of UCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13705200	KTE70038	48 T	$359		Add to Cart
BHE13705200	KTE70038	96 T	$619		Add to Cart
BHE13705200	KTE70038	96 Tx5	$2809		Add to Cart
BHE13705200	KTE70038	96 Tx50	$26209		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.