Mouse MIP-3 Alpha/CCL20 ELISA Kit PicoKine®

Mfr.No.

EK0454

Sensitivity

<1 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.023	0.201	0.394	0.761	1.251	1.852	1.996	2.090

Description

Mouse MIP-3 Alpha/CCL20 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl20 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 20;Beta-chemokine exodus-1;CC chemokine LARC;CC chemokine ST38;Liver and activation-regulated chemokine;Macrophage inflammatory protein 3 alpha;MIP-3-alpha;Small-inducible cytokine A20;Ccl20;Larc, Scya20;

Molecular Weight

10826 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A28-M97

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CCL20

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	9	55	313	9	57	308
Standard deviation	0.62	3.02	21.28	0.78	3.7	21.56
CV (%)	6.9%	5.5%	6.8%	8.7%	6.5%	7%

Protein Function

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. .

Background

Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	9	9	10	10	9	0.5	5.5%
Sample 2	55	56	52	51	53	2.06	3.8%
Sample 3	313	301	271	296	295	15.3	5.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20297
Gene Name	CCL20
UniProt ID	O89093
Protein Name	C-C motif chemokine 20
Tissue Specificity	Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues.

Applications	ELISA
Research Area	Chemokines,G Protein Signaling,Immunology,Innate Immunity,Macrophage/Inflammation,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000449	EK0454	96 wells/kit	$499		Add to Cart

Mouse MIP-3 Alpha/CCL20 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse MIP-3 Alpha/CCL20 ELISA Kit PicoKine®

Rate it now..!!