Mouse Microtubule-associated Protein 1 Light Chain 3 Alpha

Mfr.No.

E1586Mo

Description

This sandwich kit is for the accurate quantitative detection of Mouse Microtubule-associated Protein 1 Light Chain 3 Alpha (also known as MAP1LC3A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Autophagy-related protein LC3 A;Autophagy-related ubiquitin-like modifier LC3 A;MAP1 light chain 3-like protein 1;MAP1A/MAP1B LC3 A;MAP1A/MAP1B light chain 3 A;MAP1LC3A;Microtubule-associated protein 1 light chain 3 alpha;Microtubule-associated proteins 1A/1B light chain 3A

Reactivity

Mouse

Detection Range

10-4000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

14272

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.24ng/L

Background

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Gene ID	66734
UniProt ID	Q91VR7
Antigen	MAP1LC3A

Research Area

Others

Protocol

These standard curves of E1586Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.157	2.263	2.212
	2.369
1200ng/L	1.734	1.765	1.713
	1.795
600ng/L	1.222	1.27	1.219
	1.318
300ng/L	0.641	0.691	0.64
	0.741
150ng/L	0.428	0.413	0.362
	0.399
0ng/L	0.041	0.051	0
	0.062

Precision

We measured random samples of E1586Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	156.8	7.05	4.5
Intra-Assay	2	18	514.1	34.19	6.7
Intra-Assay	3	18	649.3	25.49	3.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109429	E1586Mo	48T	$320		Add to Cart
BHE12109429	E1586Mo	96T	$458		Add to Cart

Mouse Microtubule-associated Protein 1 Light Chain 3 Alpha - MAP1LC3A ELISA Kit

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