Mouse MCP-1 ELISA Kit PicoKine®

Mfr.No.

EK0568

Sensitivity

<6 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.014	0.051	0.090	0.193	0.406	0.726	1.465	1.897

Description

Mouse MCP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 2;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Platelet-derived growth factor-inducible protein JE;Small-inducible cytokine A2;Ccl2;Je, Mcp1, Scya2;

Molecular Weight

16326 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-R96

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse MCP-1

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	39	224	394	40	231	398
Standard deviation	1.56	10.75	27.18	2	12.47	27.46
CV (%)	4%	4.8%	6.9%	5%	5.4%	6.9%

Protein Function

Chemotactic factor that attracts monocytes, but not neutrophils.

Background

Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	39	36	34	34	35	2.04	5.8%
Sample 2	224	228	228	219	224	3.69	1.6%
Sample 3	394	375	431	394	398	20.3	5.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20296
Antigen	MCP-1
Gene Name	CCL2
UniProt ID	P10148
Protein Name	C-C motif chemokine 2

Applications	ELISA
Research Area	Angiogenesis,Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Chemokines,Heart Disease,Immunology,Innate Immunity,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000523	EK0568	96 wells/kit	$499		Add to Cart

Mouse MCP-1 ELISA Kit PicoKine®

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