Mouse MBL2/MPB-C ELISA Kit PicoKine®

Mfr.No.

EK0806

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.036	0.200	0.354	0.576	1.084	1.599	1.802	2.085

Description

Mouse MBL2/MPB-C ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mbl2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Mannose-binding protein C;MBP-C;Mannan-binding protein;RA-reactive factor P28A subunit;RARF/P28A;Mbl2;

Molecular Weight

25957 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-D244

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse MBL2

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	656	1840	9232	637	1954	8997
Standard deviation	32.8	84.64	618.54	42.04	111.37	665.77
CV (%)	5%	4.6%	6.7%	6.6%	5.7%	7.4%

Protein Function

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity). .

Background

MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be patte recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate pattes, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	656	711	654	663	671	23.33	3.4%
Sample 2	1840	1861	1847	1853	1850	7.72	0.4%
Sample 3	9232	10414	9072	9277	9498	533.88	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17195
Gene Name	MBL2
UniProt ID	P41317
Protein Name	Mannose-binding protein C
Sequence	Contains 1 C-type lectin domain.

Applications	ELISA
Research Area	Complement,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000969	EK0806	96 wells/kit	$499		Add to Cart

Mouse MBL2/MPB-C ELISA Kit PicoKine®

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