Mouse Marapsin/Pancresin ELISA Kit PicoKine®

Mfr.No.

EK1481

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.005	0.074	0.183	0.309	0.618	1.201	1.794	2.133

Description

Mouse Marapsin/Pancresin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Prss27 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serine protease 27;3.4.21.-;Marapsin;Pancreasin;Prss27;Mpn;

Molecular Weight

35789 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M38-V328

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Marapsin

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	178	1127	4357	171	1106	3990
Standard deviation	9.96	87.6%5	243.99	9.57	98.43	299.25
CV (%)	7.6%%	7.6%	5.6%	5.6%	8.9%	7.5%

Background

Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	178	167	199	181	181	11.49	6.3%
Sample 2	1127	1176	1034	1195	1133	62.3	5.4%
Sample 3	4357	5001	4832	4935	4781	252.23	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	213171
Gene Name	Prss27
UniProt ID	Q8BJR6
Protein Name	Serine protease 27

Applications	ELISA
Research Area	Cell Biology,Proteolysis/Ubiquitin,Proteolytic Enzymes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001076	EK1481	96 wells/kit	$499		Add to Cart

Mouse Marapsin/Pancresin ELISA Kit PicoKine®

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