Mouse Major Centromere Autoantigen B

Mfr.No.

E2232Mo

Alternative Names

CENPB;CENP-B;Centromere protein B;Major centromere autoantigen B

Reactivity

Mouse

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

65381

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.45ng/L

Background

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Gene ID	12616
UniProt ID	P27790
Antigen	CENPB

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E2232Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.978	1.968	1.929
	1.958
800ng/L	0.865	0.914	0.875
	0.962
400ng/L	0.481	0.449	0.41
	0.416
200ng/L	0.283	0.283	0.244
	0.283
100ng/L	0.148	0.15	0.111
	0.152
0ng/L	0.039	0.039	0
	0.04

Precision

We measured random samples of E2232Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	488.6	27.34	5.6
Intra-Assay	2	18	1089.3	25.59	2.3
Intra-Assay	3	18	837.4	37.45	4.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110043	E2232Mo	48T	$320		Add to Cart
BHE12110043	E2232Mo	96T	$458		Add to Cart

Mouse Major Centromere Autoantigen B - CENPB ELISA Kit

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