Mouse Lysosomal Pro-X Carboxypeptidase

Mfr.No.

E2225Mo

Alternative Names

Lysosomal Pro-X carboxypeptidase;PRCP;Proline carboxypeptidase;Prolylcarboxypeptidase

Reactivity

Mouse

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

55027

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.57ng/L

Background

Cleaves C-terminal amino acids linked to proline in Peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH (By similarity).

Gene ID	72461
UniProt ID	Q7TMR0
Antigen	PRCP

Research Area

Cardiovascular

Protocol

These standard curves of E2225Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.184	2.219	2.178
	2.253
1200ng/L	1.379	1.28	1.239
	1.181
600ng/L	0.558	0.649	0.609
	0.741
300ng/L	0.362	0.403	0.363
	0.444
150ng/L	0.145	0.175	0.135
	0.205
0ng/L	0.049	0.04	0
	0.032

Precision

We measured random samples of E2225Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1747.9	56.84	3.3
Intra-Assay	2	18	422.8	21.7	5.1
Intra-Assay	3	18	918.9	47.63	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110036	E2225Mo	48T	$320		Add to Cart
BHE12110036	E2225Mo	96T	$458		Add to Cart

Mouse Lysosomal Pro-X Carboxypeptidase - PRCP ELISA Kit

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