Mouse LRP8(Low Density Lipoprotein Receptor Related Protein 8) ELISA Kit

Mfr.No.

ELK9989

Sensitivity

15.2pg/mL

Detection Range

31.25-2000 pg/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LRP8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	92-105%	83-92%	85-96%	78-90%
EDTA Plasma(n=5)	88-97%	87-103%	81-93%	79-92%
Heparin Plasma(n=5)	89-105%	90-101%	87-93%	95-106%

Standard Curve

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.079	1.992
1000.00	1.515	1.428
500.00	1.118	1.031
250.00	0.813	0.726
125.00	0.577	0.490
62.50	0.357	0.270
31.25	0.121	0.034
0.00	0.087	0.000

Sample Type

Serum, Plasma and other biological fluids.

Limit Of Detection

2000 pg/mL

Precision

Matrices listed below were spiked with certain level of recombinant LRP8 and the recovery rates were calculated by comparing the measured value to the expected amount of LRP8 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA Plasma(n=5)	96-108%	102%
Heparin Plasma(n=5)	95-107%	101%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LRP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LRP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LRP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LRP8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

UniProt ID	Q924X6

Research Area	Metabolic pathway;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15210467	ELK9989	48T	$320		Add to Cart
BHE15210467	ELK9989	96T	$458		Add to Cart

Mouse LRP8(Low Density Lipoprotein Receptor Related Protein 8) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse LRP8(Low Density Lipoprotein Receptor Related Protein 8) ELISA Kit

Rate it now..!!