Mouse LIF ELISA Kit PicoKine®

Mfr.No.

EK0580

Sensitivity

<10 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.004	0.104	0.163	0.285	0.654	0.857	1.472	2.006

Description

Mouse LIF ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lif in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Leukemia inhibitory factor;LIF;Differentiation-stimulating factor;D factor;Lif;

Molecular Weight

22287 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-F203

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse LIF

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	16	70	272	15	76	278
Standard deviation	0.7	3.01	19.58	0.72	3.34	24.74
CV (%)	4.4%	4.3%	7.2%	4.8%	4.4%	8.9%

Protein Function

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Background

Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southe analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	16	15	16	15	15	0.5	3.3%
Sample 2	70	65	74	67	69	3.39	4.9%
Sample 3	272	305	287	293	289	11.88	4.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16878
Gene Name	Lif
UniProt ID	P09056
Protein Name	Leukemia inhibitory factor

Applications	ELISA
Research Area	Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Differentiation,Cytokines,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Neurogenesis,Neurology Process,Neuroscience,Transcription,Tumor Suppressors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001140	EK0580	96 wells/kit	$499		Add to Cart

Mouse LIF ELISA Kit PicoKine®

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