Mouse Leucine-rich Repeat-containing G-protein Coupled Receptor 5

Mfr.No.

E2015Mo

Sensitivity

9.93ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Leucine-rich repeat-containing G-protein Coupled Receptor 5 (also known as LGR5) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

G-protein coupled receptor 49;Leucine-rich repeat-containing G-protein coupled receptor 5;LGR 5;LGR5;Orphan G-protein coupled receptor FEX

Molecular Weight

99666

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2015Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	674.6	22.5	3.3
Intra-Assay	2	18	578.1	33.58	5.8
Intra-Assay	3	18	215.9	9.5	4.4

Background

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Protocol

These standard curves of E2015Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.268	2.301	2.253
	2.333
1200ng/L	1.927	1.899	1.851
	1.871
600ng/L	1.244	1.164	1.115
	1.084
300ng/L	0.685	0.683	0.635
	0.681
150ng/L	0.377	0.362	0.314
	0.348
0ng/L	0.04	0.048	0
	0.057

Gene ID	14160
UniProt ID	Q9Z1P4

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109826	E2015Mo	48T	$320		Add to Cart
BHE12109826	E2015Mo	96T	$458		Add to Cart

Mouse Leucine-rich Repeat-containing G-protein Coupled Receptor 5 - LGR5 ELISA Kit

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