Mouse Kremen-1 ELISA Kit PicoKine®

Mfr.No.

EK1538

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.044	0.072	0.151	0.291	0.591	1.121	1.838	2.415

Description

Mouse Kremen-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Kremen1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kremen protein 1;Dickkopf receptor;Kringle domain-containing transmembrane protein 1;Kringle-containing protein marking the eye and the nose;Kremen1;Kremen;

Molecular Weight

51673 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-G395

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Kremen-1

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	171	880	4010	187	831	3851
Standard deviation	6.84	57.2	308.77	8.41	59	323.48
CV (%)	4%	6.5%	7.7%	4.5%	7.1%	8.4%

Protein Function

Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity). .

Background

Kremen protein 1 is a protein that in humans is encoded by the KREMEN1 gene. This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	171	171	172	159	168	5.35	3.1%
Sample 2	880	925	969	991	941	42.6	4.5%
Sample 3	4010	4034	3878	4402	4081	194.61	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	84035
Antigen	Kremen-1
Gene Name	Kremen1
UniProt ID	Q99N43
Protein Name	Kremen protein 1
Tissue Specificity	In the adult, widely expressed with high levels in heart, lung, kidney, skeletal muscle and testis. .

Applications	ELISA
Research Area	Cadherins,Cell Adhesion,Cytoskeleton/ECM,Signal Transduction,Signaling Pathways,Stem Cells,Surface Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001117	EK1538	96 wells/kit	$750		Add to Cart

Mouse Kremen-1 ELISA Kit PicoKine®

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