Mouse Kremen-1 ELISA Kit PicoKine®

Mfr.No.

EK1538

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.044	0.072	0.151	0.291	0.591	1.121	1.838	2.415

Description

Mouse Kremen-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Kremen1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kremen protein 1;Dickkopf receptor;Kringle domain-containing transmembrane protein 1;Kringle-containing protein marking the eye and the nose;Kremen1;Kremen;

Molecular Weight

51673 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-G395

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Kremen-1

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	171	880	4010	187	831	3851
Standard deviation	6.84	57.2	308.77	8.41	59	323.48
CV (%)	4%	6.5%	7.7%	4.5%	7.1%	8.4%

Protein Function

Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity). .

Background

Kremen protein 1 is a protein that in humans is encoded by the KREMEN1 gene. This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alteatively spliced transcript variants encoding distinct isoforms have been observed for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	171	171	172	159	168	5.35	3.1%
Sample 2	880	925	969	991	941	42.6	4.5%
Sample 3	4010	4034	3878	4402	4081	194.61	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	84035
Gene Name	Kremen1
UniProt ID	Q99N43
Protein Name	Kremen protein 1
Tissue Specificity	In the adult, widely expressed with high levels in heart, lung, kidney, skeletal muscle and testis. .

Applications	ELISA
Research Area	Cadherins,Cell Adhesion,Cytoskeleton/ECM,Signal Transduction,Signaling Pathways,Stem Cells,Surface Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001117	EK1538	96 wells/kit	$750		Add to Cart

Mouse Kremen-1 ELISA Kit PicoKine®

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