Mouse KIM1 / TIM1 ELISA Kit PicoKine®

Mfr.No.

EK0880

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.036	0.061	0.104	0.172	0.294	0.520	0.977	1.756

Description

Mouse KIM1 / TIM1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Havcr1 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hepatitis A virus cellular receptor 1 homolog ;Hepatitis A virus cellular receptor 1, isoform CRA_b ;Havcr1 ;mCG_117142 ;

Molecular Weight

30968 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y22-T212

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse KIM1

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	87	279	842	85	261	792
Standard deviation	6.87	14.78	36.2	7.31	14.87	38.8
CV (%)	7.9%	5.3%	4.3%	8.6%	5.7%	4.9%

Background

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.(1)(2)(3) Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated intealization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	87	90	90	96	90	3.26	3.6%
Sample 2	279	266	249	284	269	13.53	5%
Sample 3	842	734	811	767	788	41.23	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	171283
Gene Name	HAVCR1
UniProt ID	Q3V033
Protein Name	Hepatitis A virus cellular receptor 1 homolog

Applications	ELISA
Research Area	Adaptive Immunity,Host-Virus Interaction,Immune System Diseases,Immunology,Interspecies Interaction,Microbiology,Non-CD,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000673	EK0880	96 wells/kit	$399		Add to Cart

Mouse KIM1 / TIM1 ELISA Kit PicoKine®

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