Mouse JAM-C/JAM3 ELISA Kit PicoKine®

Mfr.No.

EK1840

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.007	0.070	0.183	0.314	0.490	0.896	1.512	2.129

Description

Mouse JAM-C/JAM3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Jam3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Junctional adhesion molecule C; JAM-C; JAM-2; Junctional adhesion molecule 3; JAM-3; Jam3

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-N241

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse JAM-C/JAM3

Subcellular Localization

Cell membrane, Single-pass type I membrane protein, extracellular space, desmosome

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	58	270	884	61	279	991
Standard deviation	2.90	14.04	39.78	2.81	17.86	74.33
CV (%)	5.0%	5.2%	4.5%	4.6%	6.4%	7.5%

Protein Function

Participates in cell-cell adhesion. It is a counter-receptor for ITGAM mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angio

Background

JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 9; 9 A4. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	58	54	55	49	54	3.24	6.0%
Sample 2	270	320	291	297	294	17.81	6.0%
Sample 3	884	818	866	930	874	40.10	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	83964
Gene Name	Jam3
UniProt ID	Q9D8B7
Tissue Specificity	Endothelial cells.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cell Adhesion,Cell Adhesion Molecules,Cell Type Markers,Cytoskeleton/ECM,Platelets,Signal Transduction,Tags & Cell Markers,Vascular Inflammation,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001416	EK1840	96 wells/kit	$499		Add to Cart

Mouse JAM-C/JAM3 ELISA Kit PicoKine®

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