Mouse Insulin Receptor Substrate 1

Mfr.No.

E1163Mo

Sensitivity

0.031ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Insulin receptor substrate 1;IRS 1;IRS1;IRS-1

Molecular Weight

130723

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1163Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.5	0.21	3.9
Intra-Assay	2	18	9.1	0.28	3
Intra-Assay	3	18	2.9	0.12	4.3

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

Protocol

These standard curves of E1163Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.364	2.339	2.306
	2.314
8ng/ml	1.412	1.38	1.347
	1.348
4ng/ml	0.684	0.643	0.61
	0.601
2ng/ml	0.367	0.373	0.34
	0.38
1ng/ml	0.157	0.152	0.119
	0.146
0ng/ml	0.03	0.033	0
	0.036

Gene ID	16367
UniProt ID	P35569

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109029	E1163Mo	48T	$320		Add to Cart
BHE12109029	E1163Mo	96T	$458		Add to Cart

Mouse Insulin Receptor Substrate 1 - IRS-1 ELISA Kit

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