Mouse IL7R/CD127 ELISA Kit PicoKine®

Mfr.No.

EK0714

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.060	0.135	0.214	0.338	0.568	0.893	1.461	1.912

Description

Mouse IL7R/CD127 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il7r in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-7 receptor subunit alpha;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;CD127;Il7r;

Molecular Weight

51605 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-D239

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IL7R

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	66	270	964	61	293	970
Standard deviation	2.77	18.09	74.22	3.53	22.26	82.45
CV (%)	4.2%	6.7%	7.7%	5.8%	7.6%	8.5%

Protein Function

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

Background

The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	66	77	65	68	69	4.74	6.8%
Sample 2	270	257	287	288	275	12.85	4.6%
Sample 3	964	912	900	813	897	54.26	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16197
Gene Name	IL7R
UniProt ID	P16872
Protein Name	Interleukin-7 receptor subunit alpha
Tissue Specificity	Spleen, thymus and fetal liver.
Sequence	Belongs to the type I cytokine receptor family. Type 4 subfamily.

Applications	ELISA
Research Area	Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Interleukins,Lymphoid,Myeloid,Stem Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000916	EK0714	96 wells/kit	$499		Add to Cart

Mouse IL7R/CD127 ELISA Kit PicoKine®

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