Mouse IL15RA ELISA Kit PicoKine®

Mfr.No.

EK1142

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1,000	2,000	4,000	8,000
O.D.	0.038	0.066	0.100	0.160	0.299	0.568	1.193	2.053

Description

Mouse IL15RA ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il15ra in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-15 receptor subunit alpha;IL-15 receptor subunit alpha;IL-15R-alpha;IL-15RA;CD215;Soluble interleukin-15 receptor subunit alpha;sIL-15 receptor subunit alpha;sIL-15R-alpha;sIL-15RA;Il15ra;

Molecular Weight

28061 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G33-K205

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IL15RA

Subcellular Localization

Membrane ; Single-pass type I membrane protein . Nucleus membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	181	1121	6129	165	1015	6536
Standard deviation	12.48	77.34	306.45	11.55	86.9%6	326.8
CV (%)	6.9%	6.9%	5%	7%	8.4%	5%

Protein Function

High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Signal transduction involves SYK (By similarity). .

Background

Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	181	196	202	196	193	7.75	4%
Sample 2	1121	1352	1324	1168	1241	98.66	7.9%
Sample 3	6129	5379	6344	6290	6035	387.19	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16169
Gene Name	IL15RA
UniProt ID	Q60819
Protein Name	Interleukin-15 receptor subunit alpha
Tissue Specificity	Widely expressed. .

Applications	ELISA
Research Area	Adapters,Apoptosis,Associated Proteins,Cancer,Cell Biology,Cell Death,Metabolism,Obesity,Receptors,Signal Transduction,Transmembrane

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000788	EK1142	96 wells/kit	$499		Add to Cart

Mouse IL15RA ELISA Kit PicoKine®

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