Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®

Mfr.No.

EK0408

Sensitivity

<2 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.025	0.083	0.144	0.258	0.467	0.882	1.554	2.052

Description

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il5 in cell culture supeatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-5;IL-5;B-cell growth factor II;BCGF-II;Cytotoxic T-lymphocyte inducer;Eosinophil differentiation factor;T-cell replacing factor;TRF;Il5;Il-5;

Molecular Weight

15410 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M21-G133

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IL-5

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	36	220	453	34	214	478
Standard deviation	2.52	9.24	21.29	2.58	10.7	30.59
CV (%)	7%	4.2%	4.7%	7.6%	5%	6.4%

Protein Function

Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

Background

Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	36	31	37	35	34	2.27	6.6%
Sample 2	220	228	267	256	242	19.35	7.9%
Sample 3	453	402	397	434	421	23.07	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16191
Gene Name	IL5
UniProt ID	P04401
Protein Name	Interleukin-5

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Cancer,Cytokines,Immunology,Innate Immunity,Interleukins,Non-CD,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000416	EK0408	96 wells/kit	$499		Add to Cart

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®

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