Mouse IL-23R ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1613

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.003	0.088	0.143	0.272	0.576	1.009	1.867	2.424

Description

Mouse IL-23R ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Il23r in cell culture supeatants, serum and plasma (heparin, EDTA).

Alternative Names

Interleukin-23 receptor; IL-23 receptor; IL-23R; Il23r

Immunogen

Expression system for standard: NS0; Immunogen sequence: I25-I373

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IL-23R

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	133	607	1698	142	552	1933
Standard deviation	6.25	28.53	96.78	9.8	39.74	137.24
CV (%)	4.7%	4.7%	5.7%	6.9%	7.2%	7.1%

Protein Function

Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis (By similarity). .

Background

Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	209590
Gene Name	Il23r
UniProt ID	Q8K4B4
Tissue Specificity	Expressed by Th1, Th2 and dendritic cells.

Applications	ELISA
Research Area	Cell Type Markers,Cytokines,Epigenetics and Nuclear Signaling,Immunoglobulins,Immunology,Innate Immunity,Interleukins,Nuclear Signaling,Nuclear Signaling Pathways,Receptors,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000032	EZ1613	5 plates/kit	$500		Add to Cart

Mouse IL-23R ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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