Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine®

Mfr.No.

EK1180

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D	0.020	0.149	0.149	0.280	0.440	0.932	1.629	2.053

Description

Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il1rl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-1 receptor-like 1;Lymphocyte antigen 84;Protein ST2;Protein T1;Il1rl1;Ly84, St2, Ste2;

Molecular Weight

64801 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-R332

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IL1RL1

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	194	1525	5484	189	1538	5883
Standard deviation	8.73	94.55	296.13	10.77	115.35	394.16
CV (%)	4.5%	6.2%	5.4%	5.7%	7.5%	6.7%

Protein Function

Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function. .

Background

Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	194	192	199	206	197	5.4	2.7%
Sample 2	1525	1366	1502	1386	1444	69.58	4.8%
Sample 3	5484	5267	4842	5202	5198	230.92	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17082
Antigen	IL-1
Gene Name	IL1RL1
UniProt ID	P14719
Protein Name	Interleukin-1 receptor-like 1
Tissue Specificity	Predominantly expressed in hematopoietic tissues, and in macrophage, erythroid, epithelial and fibroblast cell lines. .
Sequence	Belongs to the interleukin-1 receptor family.

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cytokines,Hypertrophy,Immunology,Innate Immunity,Interleukins,TLR Signaling

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000802	EK1180	96 wells/kit	$499		Add to Cart

Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine®

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