Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0394

Detection Range

12.5 pg/ml - 800 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	12.5	25	50	100	200	400	800
O.D.	0.030	0.065	0.123	0.212	0.336	0.609	1.031	1.898

Description

Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Il1b in cell culture supeatants, serum and plasma (heparin, EDTA).

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V118-S269

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IL-1 beta

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Background

Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone tuover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	16176
Gene Name	Il1b
UniProt ID	P10749

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000129	EZ0394	5 plates/kit	$500		Add to Cart

Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Rate it now..!!