Matrices listed below were spiked with certain level of IgG2a and the recovery rates were calculated by comparing the measured value to the expected amount of IgG2a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
94
EDTA plasma(n=5)
87-105
97
heparin plasma(n=5)
89-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG2a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IgG2a ELISA Kit allows for the in vitro quantitative determination of IgG2a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse IgG2a(Immunoglobulin G2a) ELISA Kit