Mouse IGFBP2 ELISA Kit PicoKine®

Mfr.No.

EK0385

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.025	0.123	0.180	0.312	0.546	0.919	1.445	2.036

Description

Mouse IGFBP2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igfbp2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Insulin-like growth factor-binding protein 2;IBP-2;IGF-binding protein 2;IGFBP-2;mIGFBP-2;Igfbp2;Igfbp-2;

Molecular Weight

32847 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E35-Q305

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IGFBP2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	160	970	3281	169	968	3467
Standard deviation	7.52	76.63	203.42	8.95	86.15	256.55
CV (%)	4.7%	7.9%	6.2%	5.3%	8.9%	7.4%

Protein Function

Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

Background

Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	160	171	174	173	169	5.59	3.3%
Sample 2	970	1024	949	988	982	27.52	2.8%
Sample 3	3281	3102	2889	3320	3148	170.63	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16008
Gene Name	IGFBP2
UniProt ID	P47877
Protein Name	Insulin-like growth factor-binding protein 2
Tissue Specificity	Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. .
Sequence	Contains 1 IGFBP N-terminal domain.

Applications	ELISA
Research Area	Cancer,Extracellular Matrix,Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000395	EK0385	96 wells/kit	$499		Add to Cart

Mouse IGFBP2 ELISA Kit PicoKine®

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