Mouse Hydroxyacidoxidase 1

Mfr.No.

E2142Mo

Sensitivity

1.52ng/L

Detection Range

3-900ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Glycolate oxidase;GOX;HAO 1;HAO1;HAOX1;Hydroxyacid oxidase 1

Molecular Weight

41001

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2142Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	104.4	4.53	4.3
Intra-Assay	2	18	314.8	16.12	5.1
Intra-Assay	3	18	62.2	2.92	4.7

Background

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate (By similarity).

Protocol

These standard curves of E2142Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.231	2.138	2.083
	2.045
240ng/L	1.469	1.501	1.446
	1.533
120ng/L	0.863	0.828	0.773
	0.793
60ng/L	0.402	0.444	0.388
	0.486
30ng/L	0.298	0.272	0.217
	0.247
0ng/L	0.047	0.055	0
	0.064

Gene ID	15112
UniProt ID	Q9WU19

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109953	E2142Mo	48T	$320		Add to Cart
BHE12109953	E2142Mo	96T	$458		Add to Cart

Mouse Hydroxyacidoxidase 1 - HAO1 ELISA Kit

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