Mouse Hepatocyte Growth Factor

Mfr.No.

E0675Mo

Description

This sandwich kit is for the accurate quantitative detection of hepatocyte growth factor (also known as HGF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Hepatocyte growth factor;Hepatocyte growth factor alpha chain;Hepatocyte growth factor beta chain;Hepatopoietin-A;HGF;Scatter factor;SF

Reactivity

Mouse

Detection Range

1-300ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

82945

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.48ng/L

Background

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.

Gene ID	15234
UniProt ID	Q08048
Antigen	HGF

Research Area

Cancer

Protocol

These standard curves of E0675Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/L	2.21	2.081	2.031
	1.952
80ng/L	1.497	1.464	1.414
	1.432
40ng/L	0.764	0.735	0.685
	0.707
20ng/L	0.343	0.388	0.338
	0.433
10ng/L	0.203	0.188	0.138
	0.173
0ng/L	0.059	0.05	0
	0.042

Precision

We measured random samples of E0675Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	23.8	1.2	5
Intra-Assay	2	18	66.4	2.84	4.3
Intra-Assay	3	18	78.3	2.5	3.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108551	E0675Mo	48T	$320		Add to Cart
BHE12108551	E0675Mo	96T	$458		Add to Cart

Mouse Hepatocyte Growth Factor - HGF ELISA Kit

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