Mouse Heat Shock Factor Protein 2

Mfr.No.

E2122Mo

Alternative Names

Heat shock factor protein 2;Heat shock transcription factor 2;HSF 2;HSF2;HSTF 2

Reactivity

Mouse

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

60225

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.34ng/L

Background

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.

Gene ID	15500
UniProt ID	P38533
Antigen	HSF2

Research Area

Others

Protocol

These standard curves of E2122Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.258	2.152	2.114
	2.046
800ng/L	1.356	1.383	1.346
	1.411
400ng/L	0.837	0.896	0.858
	0.956
200ng/L	0.553	0.503	0.465
	0.452
100ng/L	0.286	0.292	0.255
	0.299
0ng/L	0.04	0.038	0
	0.035

Precision

We measured random samples of E2122Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	404	22.78	5.6
Intra-Assay	2	18	685.4	29.55	4.3
Intra-Assay	3	18	196	11.7	6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109933	E2122Mo	48T	$320		Add to Cart
BHE12109933	E2122Mo	96T	$458		Add to Cart

Mouse Heat Shock Factor Protein 2 - HSF2 ELISA Kit

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