Mouse hCG(Chorionic Gonadotropin) ELISA Kit

Mfr.No.

ELK0430

Sensitivity

0.184 mIU/mL

Detection Range

1.25-80 mIU/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

hCG; Human Chorionic Gonadotrophin

Recovery

Matrices listed below were spiked with certain level of recombinant hCG and the recovery rates were calculated by comparing the measured value to the expected amount of hCG in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	80-95%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hCG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-92%	79-96%	89-100%	85-96%
EDTA plasma(n=5)	81-94%	93-107%	93-102%	91-105%
Heparin plasma(n=5)	92-101%	82-98%	89-97%	85-93%

Typical Data

Concentration (mIU/mL)	OD	Corrected OD
80.00	1.948	1.851
40.00	1.527	1.430
20.00	1.240	1.143
10.00	0.796	0.699
5.00	0.583	0.486
2.50	0.312	0.215
1.25	0.207	0.110
0.00	0.097	0.000

Limit Of Detection

80 mIU/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse hCG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse hCG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse hCG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse hCG in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208815	ELK0430	48T	$265		Add to Cart
BHE15208815	ELK0430	96T	$376		Add to Cart

Mouse hCG(Chorionic Gonadotropin) ELISA Kit

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