Mouse Glypican-3 - GPC-3 ELISA Kit

Mfr.No.

E1101Mo

Alternative Names

AI323605;Ca;Calcium-activated neutral proteinase 3;Calpain L3;Calpain p94;Calpain-3;CANP 3;Cap;Capa-3;GPC-3;Lp;Lp82;Muscle-specific calcium-activated neutral protease 3;p9;p94

Reactivity

Mouse

Detection Range

1-400ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

94242

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.55ng/mL

Background

Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-410'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53.

Gene ID	12335
UniProt ID	Q64691
Antigen	GPC-3

Research Area

Stem Cells

Protocol

These standard curves of E1101Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/mL	2.259	2.183	2.138
	2.106
120ng/mL	1.329	1.3	1.255
	1.272
60ng/mL	0.826	0.773	0.729
	0.721
30ng/mL	0.453	0.437	0.392
	0.421
15ng/mL	0.249	0.21	0.165
	0.171
0ng/mL	0.046	0.045	0
	0.044

Precision

We measured random samples of E1101Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	98.61	4.79	4.86
Intra-Assay	2	18	133.56	3.17	2.37
Intra-Assay	3	18	35.81	1.87	5.22

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108968	E1101Mo	48T	$320		Add to Cart
BHE12108968	E1101Mo	96T	$458		Add to Cart

Mouse Glypican-3 - GPC-3 ELISA Kit

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