Mouse glucoprotein 130,gp130 ELISA KIT

Mouse glucoprotein 130,gp130 ELISA KIT

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Mfr.No.

CSB-E04572m

Sensitivity

7.81 pg/ml

Detection Range

31.25 pg/ml -2000 pg/ml

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

interleukin 6 signal transducer (gp130, oncostatin M receptor), Il6stInterleukin-6 receptor subunit beta ELISA Kit; IL-6 receptor subunit beta ELISA Kit; IL-6R subunit beta ELISA Kit; IL-6R-beta ELISA Kit; IL-6RB ELISA Kit; Interleukin-6 signal transducer ELISA Kit; Membrane glycoprotein 130 ELISA Kit; gp130 ELISA Kit; Oncostatin-M receptor subunit alpha ELISA Kit; CD antigen CD130 ELISA Kit

Recovery

The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-94
EDTA plasma (n=4)	99	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	103
	Range %	97-105
1:4	Average %	95
	Range %	90-100
1:8	Average %	94
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.178	2.098	2.138	1.977
200	1.562	1.644	1.603	1.442
100	1.138	1.118	1.128	0.967
50	0.811	0.803	0.807	0.646
25	0.452	0.460	0.456	0.295
12.5	0.325	0.315	0.320	0.159
6.25	0.227	0.231	0.229	0.068
0	0.162	0.160	0.161	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q00560

Applications	ELISA
Research Area	IL6ST

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.