Mouse Glial Cell Line-derived Neurotrophic Factor

Mfr.No.

E0677Mo

Alternative Names

Astrocyte-derived trophic factor;ATF;GDNF;Glial cell line-derived neurotrophic factor;mGDNF

Reactivity

Mouse

Detection Range

0.5-100ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

23662

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.213ng/L

Background

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Gene ID	14573
UniProt ID	P48540
Antigen	GDNF

Research Area

Neuroscience

Protocol

These standard curves of E0677Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/L	2.288	2.193	2.14
	2.099
32ng/L	1.452	1.487	1.434
	1.523
16ng/L	0.869	0.908	0.855
	0.947
8ng/L	0.436	0.463	0.41
	0.491
4ng/L	0.272	0.267	0.213
	0.261
0ng/L	0.057	0.053	0
	0.049

Precision

We measured random samples of E0677Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.6	0.35	4.6
Intra-Assay	2	18	28.6	1.16	4.1
Intra-Assay	3	18	19.2	1.28	6.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108553	E0677Mo	48T	$320		Add to Cart
BHE12108553	E0677Mo	96T	$458		Add to Cart

Mouse Glial Cell Line-derived Neurotrophic Factor - GDNF ELISA Kit

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