Mouse Ghrelin O-Acyltransferase

Mfr.No.

E2095Mo

Alternative Names

Ghrelin O-acyltransferase;Gm171;GO;GOAT;MBOAT4;Membrane-bound O-acyltransferase domain-containing protein 4;O-acyltransferase domain-containing protein 4

Reactivity

Mouse

Detection Range

3-900ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

49879

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.49ng/L

Background

Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.

Gene ID	234155
UniProt ID	P0C7A3
Antigen	MBOAT4

Research Area

Metabolism

Protocol

These standard curves of E2095Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	1.986	2.136	2.107
	2.287
240ng/L	1.463	1.481	1.451
	1.498
120ng/L	0.688	0.717	0.687
	0.746
60ng/L	0.337	0.359	0.33
	0.381
30ng/L	0.168	0.156	0.126
	0.144
0ng/L	0.027	0.03	0
	0.033

Precision

We measured random samples of E2095Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	332.72	17.5	5.26
Intra-Assay	2	18	116.88	3.6	3.08
Intra-Assay	3	18	105.5	5.32	5.04

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109906	E2095Mo	48T	$320		Add to Cart
BHE12109906	E2095Mo	96T	$458		Add to Cart

Mouse Ghrelin O-Acyltransferase - MBOAT4 ELISA Kit

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